Duplex formation of complementary oligoribonucleotides corresponding to the dihydrouridine loop neck region of several transfer ribonucleic acids

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Abstract
Formation of short, double-stranded RNA helices by the complementary oligoribonucleotides GpApGpC, GpCpYpC and ApGpCpUpC was established by the temperature-dependent changes in UV hypochromicity and circular dichroism spectra. These studies provide additional thermodynamic data on duplex formation. Duplexes .**GRAPHIC**. are especially significant as they correspond to a natural sequence, the dihydrouridine loop neck region, common to several tRNA molecules, correspond to a proposed, partial recognition site for yeast phenylalanyl-tRNA ligase (EC 6.1.1.20), and represent the 1st demonstration of a duplex with 4 base pairs, 1 of which is an A-U pair. These duplexes should serve as models for studies on tRNA-ligase interaction.