Studies on the bipolar argECBH operon of E. coli: Characterization of restriction endonuclease fragments obtained from λdargECBH transducing phages and a ColE1 argECBH plasmid
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 151 (2) , 161-168
- https://doi.org/10.1007/bf00338690
Abstract
The isolation of a new type of λ transducing phage carrying the bipolar argECBH operon of E. coli K12 is described. The argECBH segment is inserted in the phage in a direction which is opposite from that of previously isolated argECBH-carrying phages. A colE1 argECBH plasmid has been constructed. DNA fragments resulting from digestion of these genetic elements with Eco RI and HindIII restriction enzymes have been characterized by agarose gel electrophoresis and electron microscopy, including heteroduplex analysis. Two fragments are of special significance for studies on the control of arginine synthesis, one of length 9.8 kilobases carrying the whole argECBH region, the other of length 2 kilobases carrying most or all of the control region between argE and argC.Keywords
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