Acholeplasma morum, a New Non-Sterol-Requiring Species

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Abstract
Two acholeplasmas recovered from contaminated cell cultures or commercial fetal bovine serum were similar in biochemical and serological properties. The organisms, although not requiring serum or cholesterol for growth, did require a supplement of 0.5% albumin and 10 .mu.g of palmitic acid/ml to a serum-free medium for sustained growth in the absence of sterols. The growth of both organisms was inhibited in medium containing 20% horse serum, although smaller amounts of horse serum or 20% fetal bovine serum did not exert inhibitory effects. The 2 strains possessed biochemical properties typical of other non-sterol-requiring members of the Mycoplasmatales and they were serologically distinct from 7 established Acholeplasma spp. On the basis of these findings and other morphological, biological and serological properties of the organisms, it is proposed that these organisms represent a new species, A. morum. Strain 72-043 (=ATCC 33211) is the type strain.

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