Translational readthrough of the PDE2 stop codon modulates cAMP levels in Saccharomyces cerevisiae
Open Access
- 28 February 2002
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 43 (3) , 641-652
- https://doi.org/10.1046/j.1365-2958.2002.02770.x
Abstract
Summary: The efficiency of translation termination in yeast can vary several 100‐fold, depending on the context around the stop codon. We performed a computer analysis designed to identify yeast open reading frames (ORFs) containing a readthrough motif surrounding the termination codon. Eight ORFs were found to display inefficient stop codon recognition, one of which, PDE2, encodes the high‐affinity cAMP phosphodiesterase. We demonstrate that Pde2p stability is very impaired by the readthrough‐dependent extension of the protein. A 20‐fold increase in readthrough of PDE2 was observed in a [PSI+] as compared with a [psi–] strain. Consistent with this observation, an important increase in cAMP concentration was observed in suppressor backgrounds. These results provide a molecular explanation for at least some of the secondary phenotypes associated with suppressor backgrounds.Keywords
This publication has 72 references indexed in Scilit:
- Translation termination efficiency can be regulated in Saccharomyces cerevisiae by environmental stress through a prion-mediated mechanismThe EMBO Journal, 1999
- Isolation and characterization of a novel actin filament-binding protein from Saccharomyces cerevisiaeOncogene, 1998
- Ubiquitination Mediated by the Npi1p/Rsp5p Ubiquitin-protein Ligase Is Required for Endocytosis of the Yeast Uracil PermeaseJournal of Biological Chemistry, 1996
- The Efficiency of Translation Termination is Determined by a Synergistic Interplay Between Upstream and Downstream Sequences inSaccharomyces cerevisiaeJournal of Molecular Biology, 1995
- A mutant allele of the SUP45 (SAL4) gene of Saccharomyces cerevisiae shows temperature-dependent allosuppressor and omnipotent suppressor phenotypesCurrent Genetics, 1995
- The pde2 gene of Saccharomyces cerevisiae is allelic to rcal and encodes a phosphodiesterase which protects the cell from extracellullar cAMPFEBS Letters, 1993
- The proteasome/multicatalytic—multifunctional proteinase In vivo function in the ubiquitin‐dependent N‐end rule pathway of protein degradation in eukaryotesFEBS Letters, 1992
- Saccharomyces cerevisiae elongation factor 2 is phosphorylated by an endorenous kinaseFEBS Letters, 1991
- The signal for a leaky UAG stop codon in several plant viruses includes the two downstream codonsJournal of Molecular Biology, 1991
- Yeast amber suppressors corresponding to tRNA3Leu genesJournal of Molecular Biology, 1984