Relationships Among Raffinose Plasmids Determined by the Immunochemical Cross-reaction of their -Galactosidases
Open Access
- 1 October 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 114 (2) , 477-481
- https://doi.org/10.1099/00221287-114-2-477
Abstract
Summary: Plasmid-encoded α-galactosidase served as a marker enzyme for the recognition and comparison of raffinose (Raf) plasmids present in strains of Escherichia coli. Immunochemical relationships were established among Raf plasmids of 39 independent isolates from man and domestic animals (from three continents) by using antiserum against α-galactosidase. Immunodiffusion revealed three serological subclasses of α-galactosidase, which are correlated with the biological and geographical origin of the host strains. It is concluded that the raf determinants of all Raf plasmids tested have evolved from a common ancestor.This publication has 6 references indexed in Scilit:
- Characterization of plasmids that encode for the K88 colonization antigenInfection and Immunity, 1978
- Fermentation of raffinose by lactose-fermenting strains of Yersinia enterocolitica and by sucrose-fermenting strains of Escherichia coliJournal of Clinical Microbiology, 1978
- Properties of two conjugative plasmids mediating tetracycline resistance, raffinose catabolism and hydrogen sulfide production in Escherichia coliMolecular Genetics and Genomics, 1978
- Properties of pPE1572(Hys-Raf), a Plasmid Governing Hydrogen Sulphide Production and Raffinose Fermentation in Escherichia coliJournal of General Microbiology, 1977
- Raffinose Metabolism in Escherichia coli K12European Journal of Biochemistry, 1976
- Analysis of Melibiose Mutants Deficient in α-Galactosidase and Thiomethylgalactoside Permease II in Escherichia coli K-12Journal of Bacteriology, 1968