Specific125I labeling of D1 (herbicide‐binding protein)
- 1 January 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 210 (1) , 71-76
- https://doi.org/10.1016/0014-5793(87)81300-5
Abstract
When125I−was given as an artificial electron donor to non‐O2‐evolving thylakoids of spinach, a 29 kDa polypeptide was specifically tagged by125I due to its photooxidation by PS II [(1985) Plant Cell Physiol. 26, 1093–1100]. We examined precisely the125I‐labeling pattern in comparison with azido[14C]atrazine photoaffinity labeling of D1 and immunoblotting with anti‐D1 and anti‐D2, and found that D1 (herbicidebinding protein) of PS II reaction center complex is specifically tagged by125I in three different species of higher plants (spinach, pea and wheat) and a thermophilic cyanobacterium (Synechococcus vulcanus). It was suggested that D1 bears the photooxidation site or has a domain very close to the photooxidation site on the donor side of PS II, in addition to the well established binding site for Qband herbicides on the acceptor side of PS II.Keywords
This publication has 22 references indexed in Scilit:
- Characterization of O2 evolution by a wheat photosystem II reaction center complex isolated by a simplified method: Disjunction of secondary acceptor quinone and enhanced Ca2+ demandArchives of Biochemistry and Biophysics, 1986
- Simple and discrete isolation of an O2‐evolving PS II reaction center complex retaining Mn and the extrinsic 33 kDa proteinFEBS Letters, 1985
- Properties of a peripheral 34 kDa protein in Synechococcus vulcanus Photosystem II particles. Its exchangeability with spinach 33 kDa protein in reconstitution of O2 evolutionBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1985
- An oxygen‐evolving complex with a simple subunit structure ‐ ‘a water‐plastoquinone oxidoreductase” ‐ from the thermophilic cyanobacteriumSynechococcussp.FEBS Letters, 1985
- The oxygen‐evolving photosystem II core complexFEBS Letters, 1985
- Isolation of a photosystem 2 preparation from higher plants with highly enriched oxygen evolution activityFEBS Letters, 1983
- Polypeptide composition of a Photosystem II core complex. Presence of a herbicide-binding proteinBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1983
- Photoaffinity labeling of an herbicide receptor protein in chloroplast membranesProceedings of the National Academy of Sciences, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Photooxidation of ferrocyanide and iodide ions and associated phosphorylation in NH2OH-treated chloroplastsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1974