Characterization of Carrot Cell Wall Protein

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Abstract
Antiserum was raised against a synthetic nona-peptide which was predicted to have considerable homology with the unhydroxylated, unglycosylated precursor of cell wall proteins from several plants [unspecified]. The antiserum is able to recognize the major cell wall protein of incubated carrot (D. carota) root discs which is produced when the discs are treated with a proline hydroxylase inhibitor and then labeled with radioactive proline. This technique has potential applications in studying cell wall biosynthesis and its regulatory control mechanisms.