Immersion fixation of rapidly frozen, untreated tissues and suspensions of cells including spermatozoa
- 1 July 1975
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 104 (2) , 121-125
- https://doi.org/10.1111/j.1365-2818.1975.tb04011.x
Abstract
Small pieces of tissue, and cell suspensions in plastic artificial insemination (AI) ‘straws’, were frozen rapidly in Freon-12 at — 155°C, without pre-treatment. Peripheral fragments were thawed directly in 2% glutaraldehyde at 0°C, and processed for transmission electron microscopy (TEM) studies. Preservation of ultrastructure was satisfactory, and freezing artifacts were minimal.Keywords
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