In-gel digestion for mass spectrometric characterization of proteins and proteomes
Top Cited Papers
- 1 December 2006
- journal article
- research article
- Published by Springer Nature in Nature Protocols
- Vol. 1 (6) , 2856-2860
- https://doi.org/10.1038/nprot.2006.468
Abstract
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.Keywords
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