QUANTITATIVE DIFFERENCES IN PHOSPHOLIPIDS OF EJACULATED SPERMATOZOA AND SPERMATOZOA FROM THREE LEVELS OF THE EPIDIDYMIS OF THE BOAR

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Abstract
Five major phospholipids of ejaculated and epldidymal porcine spermatozoa were separated and quantitatively determined by thin-layer chromatography. These were in the order of decreasing concentration, phosphatidyl choline, phosphatidyl ethanolamine, ethanolamine plasmalogen, sphingo-myelin and choline plasmalogen. Phosphatidyl serine, lysolecithin and possibly phosphatidyl inositol were also present, but in low concentrations. The concentration of phosphatidyl choline and phosphatidyl ethanolamine in spermatozoa progressively decreased from the head to the tail of the epididymis, and was lowest in ejaculated semen. The plaflmalogen content of spermatozoa from all levels of the reproductive tract of the boar was low and varied very slightly.