Determination of Nifedipine in Human Plasma by Gas Chromatograph Equipped with Flame Thermionic Detector (FTD)

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Abstract
A simplified method for the monitoring of nifedipine (NF) plasma concentration in patients after therapeutic doses was developed by use of a gas chormatograph equipped with a flame thermionic detector (FTD). After extraction from plasma with benzene, the organic layer was evaporated and the residue was dissolved in methanol. An aliquot of the solution was injected into gas chromatography with OV-17 [methyl siloxane polymer] (3%) column. Diltiazem hydrochloride was used as an internal standard (I.S.). Enough accuracy and precision were obtained in the range of 20-300 ng/ml of plasma NF levels. The detection limit for plasma NF was about 10 ng/ml, which was almost minimum concentration in the usual dosage regimen. This method could be useful not only to monitor the concentration in the plasma but to study the pharmacokinetics of NF in human.

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