Molecular Integrity of Plant Ribosomal Ribonucleic Acid
- 1 March 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 57 (3) , 410-414
- https://doi.org/10.1104/pp.57.3.410
Abstract
Thermal denaturation of plant [pea and artichoke] ribosomal RNA followed by gel fractionation shows that although a large percentage of molecules contain breaks in the polynucleotide chain, 25S and 18S RNAs do exist as unique molecular species. Values for the rate constant of hydrolysis under routine denaturing conditions are of the order of 10-7 to 10-8 sec-1 and these are shown not to be a result of ribonuclease activity. This high rate of hydrolysis and the use of insensitive fractionation procedures may account for the reported absence of a 25S rRNA molecule and its apparent conversion to a molecule similar in size to 18S RNA.This publication has 14 references indexed in Scilit:
- Gel electrophoresis of RNA under denaturing conditionsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Occurrence of heat-dissociable ribosomal RNA in insects: The presence of three polynucleotide chains in 26 S RNA from cultured Aedes aegypti cellsJournal of Molecular Biology, 1973
- Specific dissociation of pea 25-S rRNA by hot-phenol treatmentBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Lability of 26 S ribosomal RNA in Tetrahymena pyriformisExperimental Cell Research, 1971
- RNA breakdown accompanying the isolation of pea root microsomes An analysis by polyacrylamide gel electrophoresisBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
- Polynucleotide Chain Lengths of Rapidly Labelled and Ribosomal RNA of Mammalian Cells and E. coliNature, 1969
- Characterization of a new low molecular weight RNA in HeLa cell ribosomesJournal of Molecular Biology, 1968
- Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liverBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- The estimation of polynucleotide chain length by a chemical methodBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965
- Kinetics of Heat Inactivation of Ribonucleic Acid of Tobacco Mosaic VirusNature, 1958