Calcein as a Fluorescent Marker of Otoliths of Larval and Juvenile Fish
- 1 July 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Transactions of the American Fisheries Society
- Vol. 116 (4) , 668-670
- https://doi.org/10.1577/1548-8659(1987)116<668:caafmo>2.0.co;2
Abstract
Calcein is a fluorescent compound that can bind with alkaline earth metals, such as calcium. Upon binding, an increase in fluorescence under ultraviolet light results. Therefore, calcein was evaluated as potentially useful for creating a fluorescent mark in the otoliths of larval and juvenile fish. Such a mark was produced in the sagittae of larvae and juveniles of three species of estuarine fishes after a 2-h immersion in a solution of 125 mg/L in seawater. The mark was easily distinguished under ultraviolet epifluorescent microscopy. This technique provides an alternative to the use of tetracycline in age and growth studies.This publication has 2 references indexed in Scilit:
- The Forgotten Requirement for Age Validation in Fisheries BiologyTransactions of the American Fisheries Society, 1983
- Use of Lead Compounds and Tetracycline to Mark Scales and Otoliths of Marine FishesThe Progressive Fish-Culturist, 1967