Effect of Overexpression of Actinobacillus succinogenes Phosphoenolpyruvate Carboxykinase on Succinate Production in Escherichia coli

Open Access

1 February 2004

journal article
Published by American Society for Microbiology in Applied and Environmental Microbiology

Vol. 70 (2) , 1238-1241
https://doi.org/10.1128/aem.70.2.1238-1241.2004

Abstract

Succinate fermentation was investigated in Escherichia coli strains overexpressing Actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). In E. coli K-12, PEPCK overexpression had no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant E. coli strain K-12 ppc :: kan , PEPCK overexpression increased succinate production 6.5-fold.

Keywords

This publication has 14 references indexed in Scilit:

Analysis of Escherichia coli anaplerotic metabolism and its regulation mechanisms from the metabolic responses to altered dilution rates and phosphoenolpyruvate carboxykinase knockout
Biotechnology & Bioengineering, 2003
Effects of Growth Mode and Pyruvate Carboxylase on Succinic Acid Production by Metabolically Engineered Strains of Escherichia coli
Applied and Environmental Microbiology, 2002
The physiological effects and metabolic alterations caused by the expression of Rhizobium etli pyruvate carboxylase in Escherichia coli
Applied Microbiology and Biotechnology, 2001
Metabolic flux analysis for succinic acid production by recombinant Escherichia coli with amplified malic enzyme activity
Biotechnology & Bioengineering, 2001
Molecular characterization, enzyme properties and transcriptional regulation of phosphoenolpyruvate carboxykinase and pyruvate kinase in a ruminal bacterium, Selenomonas ruminantium The GenBank accession numbers for the S. ruminantium pck and pyk sequences reported in this paper are AB016600 and AB037182, respectively.
Microbiology, 2001
Metabolic Analysis of Escherichia coli in the Presence and Absence of the Carboxylating Enzymes Phosphoenolpyruvate Carboxylase and Pyruvate Carboxylase
Applied and Environmental Microbiology, 2000
Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation by Actinobacillus sp. 130Z
Archiv für Mikrobiologie, 1997
Properties of a mutant Escherichia coli phosphoenolpyruvate carboxylase deficient in coregulation by intermediary metabolites
Journal of Bacteriology, 1981
The kinetic properties of phosphoenolpyruvate carboxykinase of Escherichia coli
Canadian Journal of Biochemistry, 1980
Propionate formation from cellulose and soluble sugars by combined cultures of Bacteroides succinogenes and Selenomonas ruminantium.
1973