Effects of growth temperature on protoplast membrane properties in Bacillus megaterium

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Abstract
Changes in the protoplast membrane of the KM strain of B. megaterium were assessed after growth at 20, 30 or 37.degree. C. Although the overall membrane concentrations of lipids and proteins were virtually unchanged, increased cultured temperature resulted in cells with membranes that contained relatively more unbranched and long-chain fatty acids and more acidic phospholipids, and different proportions and numbers of individual proteins. Electrophoretic analyses revealed 23, 31 or 29 protein bands, respectively, in membranes from cells grown at the 3 temperatures. Protoplasts from cells grown at higher temperatures were considerably less susceptible to lysis by shearing forces. As judged by passive leakage at 30.degree. C, intact cells from cultures grown at 37.degree. C were the least permeable to erythritol. Relatively low ambient concentrations of CA2+ or Mg2+ protected protoplasts from osmotic lysis but even much higher concentrations left erythritol leakage virtually unaffected. Growth temperature affected not only membrane lipids but also membrane proteins and these changes resulted in membranes with altered mechanical properties and permeabilities.

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