Abstract
The discovery of a successful method1for the cultivation ofEndameba histolyticahas given the medical profession a valuable aid in the management of amebic dysentery. The following exerpts from my experience may serve to exemplify the worth of the procedure. Patient F. C., Walter Reed General Hospital, had had amebic infection for about ten years. Several years prior to his admission, a colostomy had been performed in a civil hospital for the purpose of treatment by irrigation. Motile amebas, containing red blood cells, were present in the stool and in the colostomy wound. Study of the movements and structure of the nucleus provided additional proof that the ameba was undoubtedly E. histolytica. Culture in this case was positive in twenty-four hours, and the ameba recovered was identical with that observed in direct examinations. By subculturing every forty-eight hours, this ameba has been carried in culture for the last

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