A histochemical study about the zonal distribution of the galactose-binding protein in rat liver
- 1 January 1980
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 69 (3) , 289-297
- https://doi.org/10.1007/bf00489774
Abstract
1. Dog intestinal alkaline phosphatase (IAP), an asialoglycoprotein, appeared to be a good marker for the histochemical detection of the galactose specific binding protein in cryostat sections of rat liver. 2. Binding of IAP to the receptor is optimal at neutral or slightly alkaline pH values. The binding could be inhibited by galactose and galactose containing sugars, whereas glucose and mannose did not show any effect. In contrast to fetuin itself desialylated fetuin completely inhibited IAP binding. Pretreatment of sections with phospholipase C or with trypsin inhibited IAP binding; collagenase did not show any influence. 3. The presence of the galactose-binding protein showed a distinct zonal distribution. In the area around the central vein (zone 3) the highest IAP binding capacity was found.This publication has 20 references indexed in Scilit:
- An electron microscope autoradiographic study of the carbohydrate recognition systems in rat liver. I. Distribution of 125I-ligands among the liver cell types.The Journal of cell biology, 1979
- Unique distribution of glycoprotein receptors on parenchymal and sinusoidal cells of rat liver.Journal of Biological Chemistry, 1979
- Simultaneous localization of an hepatic binding protein specific for galactose and of galactose-containing receptors on rat hepatocytes.Journal of Histochemistry & Cytochemistry, 1978
- The uptake of native and desialylated glucocerebrosidase by rat hepatocytes and Kupffer cellsBiochemical and Biophysical Research Communications, 1978
- Hepatic Binding Protein: The Protective Role of Its Sialic Acid ResiduesScience, 1977
- Influence of fixation and buffer treatment on the release of enzymes from the plasma membraneHistochemistry and Cell Biology, 1977
- Application of enzymehistochemical methods to isolated subcellular fractions and to sucrose-ficoll density gradientsHistochemistry and Cell Biology, 1977
- The effect of phospholipase on the binding of asialoglycorproteins by rat liver plasma membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1974
- The Role of Surface Carbohydrates in the Hepatic Recognition and Transport of Circulating GlycoproteinsPublished by Wiley ,1974
- The diversity of alkaline phosphatase from rat intestine: Isolation and purification of the enzyme(s)Biochimica et Biophysica Acta (BBA) - Enzymology, 1972