Isolation of a homozygous X-linked translocation stock with two additional sex-chromosomes in the onion fly Hylemya antiqua Meigen

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Abstract
The onion fly, Hylemya antiqua Meigen, was subjected to irradiation and selection based on observations of fertility and cytogenetics, in order to isolate structural chromosome mutations which might be used for genetic control of this species. To the present time, only a “simple” X-linked translocation could be obtained as a homozygous stock. Sibcrossing was carried out using translocation trisomics (TN + X) obtained from test-crossed translocation heterozygous females (TN) showing numerical nondisjunction. A homozygous stock was obtained with two additional sex-chromosomes. This is a unique case because normally an X-linked translocation can not be made homozygous in the male sex, which normally only carries one X-chromosome.