DNA‐nuclease activity of the single‐chain ribosome‐inactivating proteins dianthin 30, saporin 6 and gelonin
- 19 August 1996
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 392 (1) , 16-20
- https://doi.org/10.1016/0014-5793(96)00776-4
Abstract
The single-chain ribosome-inactivating proteins (sc-RIPs) from plant origin are antiviral and antiproliferative agents employed in the preparation of immunotoxins. Similarly to the A-chains of ricin, sc-RIPs act as rRNA N-glycosidases. We demonstrate here that dianthin 30, saporin 6 and gelonin exert a specific nuclease activity on supercoiled DNA. Four specific sites of cleavage introduced by dianthin 30 and by saporin 6 and two specific sites of cleavage introduced by gelonin have been identified and mapped in pBR322.Keywords
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