Human Aldehyde Dehydrogenase: Mechanism of Inhibition of Disulfiram
- 7 May 1982
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 216 (4546) , 637-639
- https://doi.org/10.1126/science.7071604
Abstract
Disulfiram labeled with carbon-14 reacts specifically with human liver aldehyde dehydrogenase E1 with loss of catalytic activity and no incorporation of label. Carbon-14-labeled diethyldithiocarbamate is formed and the number of enzyme sulfhydryl groups decreases from 34 to 30 during this process.Activity is recovered by-mercaptoethanol but not by glutathione, the physiological reducing agent.Keywords
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