THE ENZYMATIC CONVERSION OF MANDELIC ACID TO BENZOIC ACID III

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Abstract
Four soluble enzymes concerned with the conversion of mandelic acid to benzoic acid were found in extracts prepared from madelate-grown cells of Pseudomonas fluorescens. They are: Mandelic acid racemase, which converts either isomer to a racemic mixture; benzoylformic carboxylase, which catalyzes a decar-boxylation of benzoylformic acid to benzaldehyde and requires cocarboxylase for its activity; and 2 benzaldehyde dehydrogenases, which react respectively with diphosphopyridine nucleotide (DPN) and triphosphopyridine nucleotide (TPN). In the light of these and preceding findings, the conversion of mandelic acid to benzoic acid can be formulated as follows:[image].