A simple, sensitive, and accurate alcohol electrode
- 1 April 1983
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 25 (4) , 1049-1055
- https://doi.org/10.1002/bit.260250413
Abstract
The construction and performance of an enzyme electrode is described which specifically detects lower primary aliphatic alcohols in aqueous solutions. The electrode consists of a commercial Clark-type oxygen electrode on which alcohol oxidase (E.C. 1.1.3.13) and catalase were immobilized. The decrease in electrode current is linearly proportional to ethanol concentrations between 1 and 25 ppm. The response of the electrode remains constant during 400 assays over a period of two weeks. The response time is between 1 and 25 min. Assembly of the electrode takes less than 1 h.Keywords
This publication has 13 references indexed in Scilit:
- Mixed substrate growth of methylotrophic yeasts in chemostat culture: Influence of the dilution rate on the utilisation of a mixture of glucose and methanolArchiv für Mikrobiologie, 1982
- The use of immobilized alcohol oxidase in the continuous flow determination of ethanol with an oxygen electrodeAnalytica Chimica Acta, 1981
- Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the alcohol oxidase.Agricultural and Biological Chemistry, 1980
- Enzymes in analytical chemistryAnalytical Chemistry, 1978
- Preparation and properties of immobilized methanol oxidaseBiotechnology & Bioengineering, 1978
- Growth of Hansenula polymorpha in a methanol-limited chemostatArchiv für Mikrobiologie, 1976
- Construction and study of electrodes using crosslinked enzymesAnalytical Chemistry, 1975
- Amperometric determination of alcohols, aldehydes and carboxylic acids with an immobilized alcohol oxidase enzyme electrodeAnalytica Chimica Acta, 1975
- Microbial Assimilation of MethanolEuropean Journal of Biochemistry, 1973
- The Microbial Metabolism of Methanol Part IIAgricultural and Biological Chemistry, 1972