Cloning and heterologous expression of a sulfur oxygenase/reductase gene from the thermoacidophilic archaeonAcidianussp. S5 inEscherichia coli
Open Access
- 1 December 2000
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 193 (2) , 217-221
- https://doi.org/10.1111/j.1574-6968.2000.tb09427.x
Abstract
A thermoacidophilic, obligately chemolithotrophic, facultatively aerobic archaebacterium, Acidianus sp. S5, was isolated from acidothermal springs in southwest China. The sulfur oxygenase/reductase (SOR) gene of Acidianus sp. S5 was cloned and expressed in Escherichia coli. Several primers were designed and successfully applied for detection and cloning of the sor gene. A 3.7-kb EcoRI fragment containing the sor gene and three neighboring open reading frames was sequenced. Sequence analysis indicated that the sor gene of Acidianus sp. S5 showed 81% identity to the sor gene of Acidianus ambivalens. E. coli cells carrying the sor gene on pBV220SOR were able to overproduce SOR upon a temperature shift from 30 to 42°C. SOR produced in E. coli catalyzes the oxidation of elemental sulfur and concomitant production of sulfite, thiosulfate and hydrogen sulfide. The recombinant enzyme exhibits the same catalytic properties as the one from Acidianus S5.Keywords
This publication has 15 references indexed in Scilit:
- 16S rDNA-based Phylogeny of the Archaeal Order Sulfolobales and Reclassification of Desulfurolobus ambivalens as Acidianus ambivalens comb. nov.Systematic and Applied Microbiology, 1996
- Molecular characterization of the sor gene, which encodes the sulfur oxygenase/reductase of the thermoacidophilic Archaeum Desulfurolobus ambivalensJournal of Bacteriology, 1992
- Oxidation of reduced inorganic sulphur compounds by acidophilic thiobacilliFEMS Microbiology Letters, 1990
- One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution.Proceedings of the National Academy of Sciences, 1989
- Coupled enzymatic production of sulfite, thiosulfate, and hydrogen sulfide from sulfur: purification and properties of a sulfur oxygenase reductase from the facultatively anaerobic archaebacterium Desulfurolobus ambivalensJournal of Bacteriology, 1989
- Methanothermus sociabilis sp. nov., a Second Species within the Methanothermaceae Growing at 97°CSystematic and Applied Microbiology, 1986
- Two contrary modes of chemolithotrophy in the same archaebacteriumNature, 1985
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Studies with cyanidium caldarium, an anomalously pigmented chlorophyteArchiv für Mikrobiologie, 1959