The Growth-Promoting Effect of Several Amino Acids on the Common Cultivated Mushroom, Agaricus bisporus

Abstract
When added to a basal medium containing glucose, asparagine, and inorganic salts, the amino acids phenylalanine, methionine, and proline were found to promote to varying degrees the growth of several strains of the white-capped form of the common cultivated mushroom, Agaricus bisporus (Lange) Singer. Phenylalanine and methionine were the most active for 2 strains while phenylalanine and proline were the most active for the other strain tested. The presence of thiamine was necessary for an appreciable growth response to these amino acids. Approximately optimal levels for the glucose, asparagine and inorganic salts present in the medium were determined in relation to the growth response to the amino-acid-vitamin mixture.