Glycosidase Activities in the Liver and Kidney of Hereditary Diabetic Mice

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Abstract
Hereditary diabetic mice (NSY) were inbred from original streptozotocin diabetic ICR mice for 8–9 generations using hyperglycemia as an index. The normoglycemic ICR mice were used as controls for the NSY line. The nonfasting blood sugar level of the NSY mice was 305±14 mg/100 ml, while their immunoreactive insulin level was 30±4 μU/ml (the values of the controls were 165±12 mg/100 ml and 79±14 μU/ml, respectively). β-N-Acetylglucosaminidase [EC 3.2.1.29], β-galastosidase [EC 3.2.1.23], α-glucosidase [EC 3.2.1.21], and α-mannosidase [EC 3.2.1.24] activities were determined in the 1,000×g supernatant of the liver and the kidney of control and streptozotocin diabetic ICR mice and their NSY line. In the kidneys of the insulinopenic NSY mice, the β-galactosidase and α-mannosidase activities were significantly decreased. No significant changes were found in liver enzyme activities. Insulin treatment increased the kidney β-galactosidase activity significantly. The insulinopenic state, which caused a decrease in the glycosidase activities in the kidney, could induce retarded breakdown of glycoprotein.

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