CHARACTERIZATION OF LECTIN-INDUCED CELLULAR CYTO-TOXICITY MEDIATED BY MOUSE SPLEEN-CELLS AND THE ROLE OF LYMPHOTOXIN

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 41  (3) , 525-534
Abstract
The cellular cytotoxicity mediated by mouse spleen cells in the presence of mitogenic or nonmitogenic lectins was established under serum-free conditions and characterized. Compared with the lectin-induced cellular cytotoxicity (LICC) in the guinea pig, the activity of lymphotoxin (LT) released into the murine LICC assay cultures was very low. A positive correlation was found between the strength of LICC and the LT activity released into the supernatants. The addition of puromycin, a potent enhancing reagent of guinea pig LT activity, markedly promoted the LICC when added 4 h after the initiation of the LICC culture. LT probably acts as an effector molecule in the murine LICC systems and in the guinea pig LICC systems. Properties of the effector cell populations mediating LICC were investigated by depletion of plastic-adherent or nylon-wool adherent cells, by treatment of spleen cells with anti-T cell sera and complement and by use of nude mouse spleen cells. Both concanavalin A and phytohemagglutinin-P apparently can induce nylon-wool non-adherent T-cell mediated LICC; phytohemagglutinin-W was capable of inducing the nylon-wool non-adherent T-cell mediated LICC and the nylon-wool adherent non-T-cell mediated LICC; the major effector of Bauhinia purpurea agglutinin-LICC was the nylon-adherent non-T-cell population.