Improved High-Performance Liquid Chromatography of Phospholipids
- 1 January 1980
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 3 (8) , 1183-1194
- https://doi.org/10.1080/01483918008064750
Abstract
The reversed-phase HPLC of phosphatidylcholine and di- and triglycerides is presented with particular emphasis on improving the efficiency of the phospholipid separations so that they are comparable to those attained for di- and triglycerides. Elevated column temperatures (60°C) and addition of strong mineral acids or ion-pairing agents to the mobile phase were found useful in accomplishing this goal.Keywords
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