Sperm Swimming Velocity as Evaluated by Frame Lapse Videography and Computer Analysis

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Abstract
A videographic method is described to measure the swimming velocity of fresh human, frozen-thawed bull and white mussel (Donax serra) sperm. The method can be accurately controlled. The pattern of sperm movement can be analyzed on a frame-by-frame basis or over 0.0206-sec intervals. Sperm velocities can be standardized per time unit. Sperm swimming velocity of different species ranging from very slow to very fast erratically moving sperm can be measured. Parameters such as swimming speed, progressive swimming speed, progressiveness ratio, and percentage motility can be evaluated.