Priming of DNA synthesis by diadenosine 5',5"'-P1,P4-tetraphosphate with a double-stranded octadecamer as a template and DNA polymerase alpha.
- 1 March 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (6) , 1791-1794
- https://doi.org/10.1073/pnas.79.6.1791
Abstract
Diadenosine 5'',5''''''-P1,P4-tetraphosphate (Ap4A) primes DNA synthesis in an in vitro system containing purified HeLa cell DNA polymerase .alpha., dATP and the double-stranded synthetic octadecamer template .**GRAPHIC**. this octadecamer sequence is part of the origin region of DNA synthesis in SV40. Ap4A is covalently linked to the 1st residue of the short deoxynucleotide chain synthesized under these experimental conditions. This template-primer system can initiate the new deoxynucleotide chain but cannot extend it beyond the A.cntdot.T region.This publication has 21 references indexed in Scilit:
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