The Dissociation of Hemolytic Antibody from Sensitized Cells as Measured by Cell-to-Cell Transfer

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Abstract
Summary: A method is presented for quantitating the extent of cell-to-cell transfer of hemolytic antibody. A number of sera obtained from rabbits immunized with either sheep cells or sheep cell stromas were examined. The differences in the percentage of transfer were found to be primarily due to differences in the percentage of readily dissociable and nondissociable antibody rather than variations in dissociation rates of the hemolytic antibody of the different sera. Electrophoretic fractionation of these sera indicated that the nondissociable hemolytic antibody was found in the γ1 electrophoretic fractions. On the other hand, the readily dissociable hemolytic antibody was found in the γ2 fractions. The differences observed in the percentage of transferable antibody appeared to correlate well with the relative amount of γ1 and γ2 hemolytic antibodies which were present. The amount of antibody transferred was directly proportional to the amount of antibody used for sensitization. This suggests that if antibody complexes are formed, such antibody complexes do not add any stability to the antigen-antibody reaction. An inverse relation was found between the percentage of transferable antibody and the 1/n value (a measure of the relationship between relative antibody concentration and percentage lysis.) The significance of this finding in regard to immune hemolysis is discussed.

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