Beef‐heart mitochondrial F1‐ATPase can use endogenous bound phosphate to synthesize ATP in dimethyl sulfoxide

Abstract
Beer-heart mitochondrial F1-ATPase contained 5 mol of inorganic phosphate bound per mol of F1, following pretreatment with ATP. A portion of the phosphate, bound most likely at a catalytic site, reacted in dimethylsulfoxide with endogenous adenine nucleotide to form ATP.