A mutation in the receiver domain of the Agrobacterium tumefaciens transcriptional regulator VirG increases its affinity for operator DNA
- 1 April 1994
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 12 (1) , 23-30
- https://doi.org/10.1111/j.1365-2958.1994.tb00991.x
Abstract
We fused the wild-type Agrobacterium tumefaciens virG gene and the constitutive virGN54D allele to the malE gene of Escherichia coli, and studied the binding of MBP-VirG fusions to the autoregulated virG promoter. MBP-VirGN54D protein bound this promoter with 10-fold higher affinity than MBP-VirG, and bound to vir box I with eightfold higher affinity than to vir box III. Disruption of vir box III did not alter the affinity for vir box I, suggesting a lack of cooperativity between these sites. We provide evidence that protein bound at a single vir box may have a higher oligomeric state than non-bound protein, and that a DNA distortion adjacent to vir box I may occur during activation.Keywords
This publication has 34 references indexed in Scilit:
- Characterization of a virG mutation that confers constitutive virulence gene expression in AgrobacteriumMolecular Microbiology, 1993
- Two-Component Sensory Transduction Systems in PhytobacteriaAnnual Review of Phytopathology, 1992
- Chronicles From the Agrobacterium-Plant Cell DNA Transfer StoryAnnual Review of Plant Biology, 1992
- Specific binding of VirG to the vir box requires a C‐ terminal domain and exhibits a minimum concentration thresholdMolecular Microbiology, 1990
- Initiation of transcription at the bacterial glnAp2 promoter by purified E. coli components is facilitated by enhancersCell, 1987
- Replacement of potassium chloride by potassium glutamate dramatically enhances protein-DNA interactions in vitroBiochemistry, 1987
- virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciensCell, 1986
- Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciensNature, 1985