Base excision repair of DNA in γ-irradiated human cells
- 1 April 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 8 (4) , 607-609
- https://doi.org/10.1093/carcin/8.4.607
Abstract
Escherichia coli endonuclease IV was used to incise cellular DNA specifically at apurinic/apyrimidinic (AP) sites prior to alkaline elution to measure the resulting DNA strand breaks. .gamma.-Irradiated HeLa cells initially contained DNA strand breaks and no AP sites. Upon incubation at 37.degree. C the strand breaks were rapidly repaired and AP sites were generated and subsequently repaired. The transient nature of the AP sites indicates the in vivo operation of a base excision repair pathway whereby damaged bases are removed from DNA by DNA glycolases to produce AP intermediates that are then substrates for AP endonucleases.Keywords
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