Biosorption of metal ions byAzotobacter vinelandii

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Abstract
Azotobacter vinelandii was better than eitherDerxia gummosa orRhizobium trifolii for sorption of UO 2+2 . Its maximum binding capacity was 0.25 mmol UO 2+2 /g dry biomass with an affinity constant of 333 l/mmol at pH 4.1 according to the Langmuir model. In a semisynthetic medium,A. vinelandii showed the highest sorption capacity in the early stationary phase. The binding of UO 2+2 , Cu2+, Ca2+ and Zn2+ was affected by the pH of the solution. With HCl as eluent, virtually all the sorbed UO 2+2 was released. The presence of Cu2+, Cd2+, Ca2+, and Zn2+ inhibited the UO 2+2 biosorption whereas Mg2+ and K+ had no effect.