Cathodoluminescence applied to immunofluorescence: Present state and improved technical prospects by prism spectrometer light selection

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Abstract
For studies on cathodoluminescence, we equipped a scanning electron microscope with a prism spectrometer and sensitive photomultiplier. The apparatus is described and our initial results are presented on the analyse of cathodoluminescence. The material observed primarily involved studies of immunofluorescent specimens. Human lymphocytes were labelled with a fluorescent antibody and cryosections of rat kidney with Masugi nephritis were labelled with a fluorescent specific antibody. Our apparatus permitted monochromatic imaging of cathodoluminescence emissions and resulted in much improved micrographs. Some possible improvements of the technique are discussed.