Determination of 2-Chloroethanol in Honey, Beeswax, and Pollen

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Abstract
Procedures were developed and tested for the determination of residues of 2-chloroethanol (ethylene chlorohydrin, ECH) in honey, beeswax, and pollen. Recoveries of ECH from fortified samples averaged 91,87, and 89%, respectively, for each substrate. The maximum amount of ECH found in substrate fumigated with ethylene oxide was 36 μg/g in honey, 124 μg/g in beeswax, and 132 μg/g in pollen. A tendency was noted for darker waxes, which contain larger amounts of naturally occurring chlorides than light-colored waxes, to contain the greater amounts of ECH. A gas-liquid chromatograph equipped with a Dohrmann halogen-specific detector was used for identification and quantitation.

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