Electron Capture Gas Chromatography Determination of Benzodiazepines and Metabolites

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Abstract
A method for the rapid qualitative and quantitative determination of five major benzodiazepine drugs in one milliliter of human plasma is described. These drugs are analyzed as the underivatized compounds by electron capture gas chromatography on a 1 meter 3% OV-17 column. By virtue of the addition of N-methyl clonazepam to the plasma prior to extraction, quantitative evaluations can be made for the individual drugs from previously prepared standard curves. The data given includes the recovery responses for the internal standard, the various drugs and their metabolites after extraction and gas chromatographic analysis. Chromatograms are illustrated for patients who had ingested the various drugs.