Biosynthesis of Sulfoquinovosyldiacylglycerol in Higher Plants

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Abstract
Adenosine-5''-phosphosulfate (APS) and adenosine-3''-phosphate 5''-phosphosulfate (PAPS) have been used as precursors of sulfoquinovosyldiacylglycerol (SQDG) in intact chloroplasts [Spinacia oleracea] incubated in the dark. Competition studies demonstrated APS was preferred over PAPS and SO42-. Rates of SQDG synthesis up to 3 nanomoles per milligram of chlorophyll per hour were observed when [35S]APS and appropriate cofactors were supplied to chloroplasts incubated in the dark. The pH optimum for utilization of APS was 7.0. The incorporation was linear for at least 30 minutes. ATP and UTP stimulated the incorporation of sulfur from APS into SQDG, but the most stimulatory additions were DHAP and glycerol-3-P. The concentration curve for APS showed a maximum at 20 micromolar in the absence of DHAP and 30 micromolar in the presence of DHAP. The optimum concentration of DHAP for conversion of APS and SQDG was 2 millimolar. Rates of synthesis up to 4 nanomoles per milligram of chlorophyll per hour were observed when [35S]PAPS was the sulfur donor and appropriate cofactors were supplied to chloroplasts. Optimal rates of conversion of sulfur from PAPS into SQDG occurred with concentrations of DHAP between 5 and 10 millimolar. DHAP was by far the most effective cofactor, although ATP and UTP also stimulated the utilization of PAPS for SQDG biosynthesis. In general, triose phosphates, including glycerol-3-P were not effective cofactors for SQDG biosynthesis.