A Semi-Automated Bone Pretreatment System and the Pretreatment of Older and Contaminated Samples
- 1 January 1989
- journal article
- Published by Cambridge University Press (CUP) in Radiocarbon
- Vol. 31 (03) , 247-253
- https://doi.org/10.1017/s0033822200011759
Abstract
A semi-automated continuous-flow system used to process archaeological bone to purified gelatin or amino acids for 14C dating is described. Powdered bone is retained in flow cells specifically designed to permit the sequential leaching of the bone with acid, alkali and water. Crude collagen obtained by this process is gelatinized, and than either purified directly using a macroporous cation exchange resin (BioRad AGMP-50), or hydrolyzed and the amino acids desalted on BioRad 50W-X8 resin. When compared with previous methods used by the laboratory, the new method allows more samples to be treated to a higher degree of purification. Examples of dates obtained on “standard” bones are presented, and confirm that no contamination is introduced from the components used in the new process.Keywords
This publication has 1 reference indexed in Scilit:
- Degradation of Amino Acids Accompanying in vitro Protein HydrolysisPublished by Springer Nature ,1985