In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions
- 1 April 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 22 (1) , 86-96
- https://doi.org/10.1128/jvi.22.1.86-96.1977
Abstract
We have analyzed the DNA products synthesized in vitro in reconstructed reactions containing purified avian oncornavirus genome RNA and RNA-directed DNA polymerase. The results of these studies indicate that: (i) the initial DNA product synthesized on either 70S RNA or reconstituted 35S RNA-tRNAtrp template - primer complexes in the presence of low concentrations of deoxynucleoside triphosphates consists of several discrete size classes, none of which exceed 200 nucleotides in length; (ii) large DNA transcripts (about 2,000 nucleotides) can be synthesized on both 70S RNA and the 35S RNA - tRNAtrp complex by increasing the deoxynucleoside triphosphate concentration; and (iii) DNA synthesized by detergent-disrupted virus is considerably longer than DNA synthesized in reconstructed reactions.This publication has 36 references indexed in Scilit:
- Ordered transcription of RNA tumor virus genomesJournal of Molecular Biology, 1976
- Specificity of in Vitro Binding of Primate Type C Viral RNA and the Homologous Viral p12 Core ProteinScience, 1976
- Initiation sites of Rous sarcoma virus RNA-directed DNA synthesis in vitroNature, 1976
- Isolation of nucleic acid-binding protein: stimulation of reverse transcriptase-catalysed DNA synthesisNature, 1976
- Specific binding of the type C viral core protein p12 with purified viral RNACell, 1976
- Blocked, methylated 5′-terminal sequence in avian sarcoma virus RNANature, 1975
- ‘Single-stranded’ DNA from φX174 and M13 is cleaved by certain restriction endonucleasesNature, 1975
- Hemophilus aegyptius restriction endonuclease cleavage map of the simian virus 40 genome and its colinear relation with the Hemophilus influenzae cleavage map of SV40Journal of Molecular Biology, 1974
- Transcription of ribonucleic acid by the ribonucleic acid directed deoxyribonucleic acid polymerase of Rous sarcoma virus and deoxyribonucleic acid polymerase I of Escherichia coliBiochemistry, 1973
- Iodination of nucleic acids in vitroBiochemistry, 1971