Alterations in PKC signaling underlie enhanced myogenic tone in exercise-trained porcine coronary resistance arteries

Publisher Website
Google Scholar
Abstract
The intracellular mechanisms underlying enhanced myogenic contraction (MC) in coronary resistance arteries (CRAs) from exercise-trained (EX) pigs have not been established. The purpose of this study was to test the hypothesis that exercise-induced alterations in protein kinase C (PKC) signaling underlie enhanced MC. Furthermore, we sought to determine whether modulation of intracellular Ca2+signaling by PKC underlies enhanced MC in EX animals. Male Yucatan miniature swine were treadmill trained ( n = 7) at ∼75% of maximal O2uptake for 16 wk (6 miles/h, 60 min) or remained sedentary (SED, n = 6). Diameter measurements in response to intraluminal pressure (60, 75, and 90 cmH2O) or 60 mM KCl were determined in single, cannulated CRAs (∼100 μm ID) with and without the PKC inhibitor chelerythrine (CE, 1 μM). Confocal imaging of Ca2+signaling [myogenic Ca2+(Cam)] was also performed in CRAs of similar internal diameter after abluminal loading of the Ca2+indicator dye fluo 4 (1 μM, 37°C, 30 min). We observed significantly greater MC in CRAs isolated from EX than from SED animals at 90 cmH2O, as well as greater reductions in MC after CE at all pressures studied. At intraluminal pressures of 75 and 90 cmH2O, CE produced greater decreases in Camin CRAs from EX than from SED animals (64% vs. 25%, P < 0.05). Inhibition of KCl constriction and Camby CE was also greater in EX animals ( P < 0.05). Western blotting revealed significant increases in Ca2+-dependent PKC-α (∼50%) but not Ca2+-independent PKC-ϵ levels in CRAs isolated from EX animals ( P < 0.05). We also observed significant group differences in phosphorylated PKC-α levels. Finally, voltage-gated Ca2+current (VGCC) was effectively blocked by CE, bisindolylmaleimide, and staurosporine in isolated smooth muscle cells from CRAs, providing evidence for a mechanistic link between VGCCs and PKC in our experimental paradigm. These results suggest that enhanced MC in CRAs from EX animals involves PKC-dependent modulation of intracellular Ca2+, including regulation of VGCCs.