Preparation and Characterization of a Murine Monoclonal Antibody (MDR3M) Reactive withmdr3Gene Product
- 26 August 1992
- journal article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 83 (8) , 795-797
- https://doi.org/10.1111/j.1349-7006.1992.tb01982.x
Abstract
A murine monoclonal antibody (MDR3M) (isotype: IgM) reactive with mdr3 gene product was generated by immunizing mice with mdr3‐specific peptide (H2N‐12WRPTSAEGDFELGISSKQKRKKTKTVKMI41G‐COOH) and hybridizing the primed mouse splenic B cells with X63‐Ag8,6.5.3 mouse plasmacytoma cells. MDR3M did not cross‐react with mdr1 gene product. This monoclonal antibody may be useful for analyzing the role of mdr3 gene product in cells and tissues.Keywords
This publication has 22 references indexed in Scilit:
- Primer‐dependent Amplification of mdr1 mRNA by Polymerase Chain ReactionJapanese Journal of Cancer Research, 1992
- Expression of mdr1 and mdr3 Multidrug-resistance Genes in Human Acute and Chronic Leukemias and Association With Stimulation of Drug Accumulation by CyclosporineJNCI Journal of the National Cancer Institute, 1990
- Expression of themdr3 gene in prolymphocytic leukemia: Association with cyclosporin-a-induced increase in drug accumulationInternational Journal of Cancer, 1990
- Immunocytochemical Identification and Localization of the Mr 22,000 Calcium‐binding Protein (Sorcin) in an Adriamycin‐resistant Myelogenous Leukemia Cell LineJapanese Journal of Cancer Research, 1989
- Sequence of mdr3 cDNA encoding a human P-glycoproteinGene, 1988
- Cellular and Tissue Distribution of MRK20 Murine Monoclonal Antibody‐defined 85‐kDa Protein in Adriamycin‐resistant Cancer Cell Lines*1Japanese Journal of Cancer Research, 1988
- Multiple-Drug Resistance in Human CancerNew England Journal of Medicine, 1987
- Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell lineNature, 1979
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975