An Immunofluorescent Staining Method for Rapid Identification of Respiratory Syncytial Virus.

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Abstract
Summary An immunofluorescence method for rapid identification of RS virus is described; results of the identification tests were available in approximately 24 hours. With respect to sensitivity, it appears that as few as 10 infectious virus particles per ml of inoculum are readily detectable within a similar period. Seventy-one of 75 viral isolates examined by the immunofluorescence method were identified as RS virus. The remaining viruses were subsequently identified as 1 mumps virus, 1 simian virus, and 2 adenoviruses. Although serum-neutralization tests indicate the existence of antigenic differences between strains of RS virus(3), no differences in antigenic composition between strains were noted in this study by immunofluorescent staining. A possible reason for this is discussed.

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