Soybean 11S globulin polypeptides have an antigenic homology with 11S globulins from various plants
- 1 July 1988
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 76 (1) , 143-147
- https://doi.org/10.1007/bf00288845
Abstract
Rabbit serum antibodies (AB) against glycinin acidic polypeptides were separated by cross exhausting, and the antibody fractions for each of the two subfamilies of glycinin subunits (A1 and A3) were obtained. The antibodies were used in the immuno blot assay with seed protein of various plant classes. Polypeptides homologous to soybean glycinin were detected. Homology with A1 polypeptide was revealed in more cases than with A3. Total seed protein preparations were subjected to centrifugation in sucrose density gradient, and the polypeptides, imunochemically related to glycinin, occurred only in fractions with sedimentation constant about 11S. The nativity of conservative antigenic determinants of 11S globulins is discussed.This publication has 18 references indexed in Scilit:
- Compartmentalization of seed reserve proteinsFEBS Letters, 1984
- The chemistry of legume storage proteinsPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1984
- Comparison of the antigenic properties of the glycinin polypeptidesArchives of Biochemistry and Biophysics, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250Analytical Biochemistry, 1977
- Legumin and vicilin, storage proteins of legume seedsPhytochemistry, 1976
- Immunochemical behaviour of legumin and vicilin from Vicia faba: a survey of related Proteins in the leguminosae subfamily faboideaeBiochemical Systematics and Ecology, 1975
- Proteins of Soybean SeedsPlant Physiology, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- An Electrophoretic Investigation of Vicilin and Legumin from Seeds of Peas.Acta Chemica Scandinavica, 1950