Expression of oncogenes during induced differentiation of human hepatocarcinoma cell line

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Abstract
There was no detectable expression of c-fos, but a little c-myc, high c-fms and mederate high IGF-I mRNA in the untreated human hepatocarcinoma cell SMMC-7721. After treatment with 10 μmol/L retinoic acid or 0.5 mmol/ L dibutyryl cyclic-3′, 5′ adenosine monophosphate (db-cAMP), the c-fos was transiently expressed within 20–60 mins. If the treatment of RA or db-cAMP prolonged to 1–5 days, the transcriptions of c-myc were increased, reaching the highest level on the 2nd and 4th day.Simultaneously the transcriptions of c-fms and IGF-I were gradually decreased. On the 5th day of the treatment, c-fms and IGF-I mRNA were decreased to 32% and 14% respectively of the control (untreated cell) value by RA, and 35% and 22% respectively by db-cAMP. The biological significance of the above mentioned results was discussed.