Some Observations on the Behavior of Vaccinia Virus Particles with Antiserum, Particularly Their Agglutination

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Abstract
Summary: The growth curve of antiserum-treated vaccinia virus in cultures of L cells resembles that from smaller inocula of untreated virus. Except when used in high concentration, the serum does not change the ultimate yield of new virus. The antibody-absorbing powers of virus preparations of high and low plaquing efficiency, as well as of those inactivated by UV, are closely related to the number of particles present, not to the number of plaque-forming units. Kinetic studies of the agglutination of vaccinia virus by antiserum, via electron microscopy, show serum strengths which are proportional to those obtained in plaque suppression experiments. Virus particles agglutinated by strong sera are often enmeshed in an amorphous cloud of unresolved structure, possibly composed of globulin molecules, which is not seen among particles agglutinated by other means.

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