Use of ‘Nuclear Monolayers’ to Identify Factors Influencing DNA Double-Strand Breakage by X-rays
- 1 January 1987
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 52 (6) , 853-858
- https://doi.org/10.1080/09553008714552441
Abstract
Nuclear monolayers, prepared by treatment of mammalian cells with non-ionic detergents, showed increased sensitivity to X-ray-induced DNA double-strand breakage (dsb), as compared with intact cells, due to a decrease in the low-dose ‘shoulder’. The DNA dsb dose–response shoulder could be restored by irradiating nuclei in the presence of sulphydryl compounds. However, the ineffectiveness of glutathione, when used at near cellular levels, in restoring the shoulder, suggested a possible role for protein sulphydryls in the radiation response of intact cells.This publication has 20 references indexed in Scilit:
- Modulation of the sensitivity of chromatin to exogenous nucleases: implications for the apparent increased sensitivity of transcriptionally-active genesBiochemistry, 1986
- Radiobiological studies with a series of human cell lines of varying glutathione contentThe British Journal of Radiology, 1984
- Transport of glutathione, as gamma-glutamylcysteinylglycyl ester, into liver and kidney.Proceedings of the National Academy of Sciences, 1983
- Peroxides in detergents as interfering factors in biochemical analysisAnalytical Biochemistry, 1977
- The detergent-resistant cytoskeleton of tissue culture cells includes the nucleus and the microfilament bundlesExperimental Cell Research, 1977
- DNA synthesis in ‘nuclear monolayers’ from BSC-1 cells infected with herpes virusNature, 1974
- Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissuesAnalytical Biochemistry, 1969