INFLUENCE OF CYSTEAMINE ON DIFFERENTIAL STAINING OF BUdR-SUBSTITUTED HUMAN CHROMOSOMES
- 1 March 1979
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Genetics and Cytology
- Vol. 21 (1) , 145-149
- https://doi.org/10.1139/g79-019
Abstract
In 5-bromodeoxyuridine (BUdR)-substituted human chromosomes stained with 4''-6-diamidino-2-phenylindole (DAPI), differential staining is suppressed totally by the H+-donor cysteamine (concentration 0.08 M). Differential staining may appear because the double BUdR-substituted chromatid is disintegrated via a photosensitive dye-visible light system. Cysteamine may prevent the production of strand breaks in DNA and, consequently, differential staining in BUdR-substituted chromosomes. Differential staining with DAPI causes irreversible changes in the double BUdR-substituted chromatid; this can be explained by the above-mentioned mechanism.This publication has 6 references indexed in Scilit:
- Factors involved in differential giemsa-staining of sister chromatidsChromosoma, 1978
- Further studies on the mechanism involved in differential staining of BUdR-substituted Vicia faba chromosomesExperimental Cell Research, 1977
- Optical studies of the interaction of 4?-6-diamidino-2-phenylindole with DNA and metaphase chromosomesChromosoma, 1977
- Mechanism of differential staining of BUdR-substituted Vicia faba chromosomesExperimental Cell Research, 1976
- Detection of sister chromatid exchanges by 4?-6-diamidino-2-phenylindole fluorescenceChromosoma, 1976
- Damage by Visible Light to the Acridine Orange-DNA ComplexBiophysical Journal, 1961