Magnetization‐transfer n.m.r. investigation of the hydrogen exchang in H20 of the peptide fragment B23‐B29 of insulin
- 1 October 1989
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 34 (4) , 340-345
- https://doi.org/10.1111/j.1399-3011.1989.tb01584.x
Abstract
Detailed and precise information on the exchanges in water of the peptide hydrogens of the insulin fragment B23-B29 (Gly23-Phe24-Phe25-Tyr26-Thr27-Pro28-Lys29) has been obtained from magnetization-transfer measurements, and nonlinear least-squares fits of the experimental spectra using the expression for the discrete Fourier transform of a sum of exponentially damped sinusoids. From a comparison of the differential exchange rates with those expected for completely solvent-exposed peptide hydrogens, specific conformational features of the heptapeptide are suggested.Keywords
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