Restoration of the Excitability of Squid Giant Axon by Tubulin—Tyrosine Ligase and Microtubule Proteins1
- 1 October 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 86 (4) , 1155-1158
- https://doi.org/10.1093/oxfordjournals.jbchem.a132611
Abstract
Membrane excitability of the squid giant axon was destroyed by perfusing with a medium containing 0.2 mM Ca ions that depolymerize microtubules. Restoration of the membrane excitability was achieved by perfusing the axon with a medium that contained purified tubulin-tyrosine ligase, tubulin, ATP, Mg2+K+, cyclic AMP, and axonal 300 K protein.Keywords
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